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Published on 02/02/2015

Glanders, equine Germany (OIE report 2015 01 30)

The affected horse was born in May 2008 and kept in the holding concerned since November 2014. On 26 November 2014 a blood sample has been taken for export purposes to a Third country with respect to glanders. The horse showed no clinical signs and has been kept separately from other horses during the pre-export quarantine. The complement fixation test showed a positive result (Land laboratory); the Friedrich-Loeffler-Institut (FLI)(National Reference Laboratory for Glanders) confirmed the result and revealed also a positive result with the immunoblot method. A second blood sample was taken and the results of this investigation confirmed the first results. As there were no epidemiological evidence for the infection the affected horse has been culled for diagnostic purposes on 13 December 2014. The bacteriological investigations of the organs showed negative results for Burkholderia mallei. The investigation of skin samples with immunohistolgical methods showed doubtful results. Further investigation of scabs of skin samples (fixed in formalin) with PCR showed positive results. The infection with Burkholderia mallei was confirmed on 27 January 2015.

Source OIE :